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Title: Variation arising through tissue culture in soft fruits
Author: Khan, Saifullah
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1997
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The study mainly concerns the potential of the tissue culture for mass propagation and to evaluate the trueness to type of regenerated plants from tissue culture using appropriate molecular markers. Three soft fruits species Ribes, Fragaria and Rubus were used in this study. The study has three main sections dealing with an evaluation of appropriate molecular marker systems to study the plants regenerated via micropropagation, callus culture and regeneration and tissue culture and regeneration. The first section evaluates the potential of SDS-PAGE protein electrophoresis and RAPD-PCR as markers to distinguish among clonally propagated cultivars of R.nigrum. SDS-PAGE was able to distinguish only four out of ten cultivars tested. RAPD-PCR was able to distinguish all the cultivars studied using only two primers. The data generated by RAPD-PCR and from pedigree information was used to examine the relatedness among the cultivars studied. RAPD-PCR was further used to examine the purity of the cultivar Baldwin collected at various locations in the UK. Polymorphism was detected and differences were found between the sub samples of a single cultivar. The second section deals with the multiplication of Rubus, Ribes and Fragaria by micropropagation. The effect of culture cycle on the plants regenerated was evaluated using RAPD-PCR. Ribes did not show any variation until the 14th generation cycle but in the 15th and 16th cycles variation was detected from 6.2% and 13.4% respectively. Considerable variation was detected in Rubus starting with the 4th subculture and was at maximum in the 7th subculture. In Fragaria, all plants at subculture 3 were evaluated and variation was detected between them. The relevance of such variation on the release of material of all the release species is discussed in relation to certification scheme requirements. The third experimental section evaluates the potential of callus as explant source for the multiplication and regeneration of plants in Ribes and Fragaria species.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available