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Title: The development and utilisation of anther culture technology in barley breeding
Author: Finnie, Susan Jane
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1990
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In this thesis, the potential of barley anther culture as a method for the rapid and efficient production of homozygous lines has been investigated. The substitution of sucrose by maltose in the anther culture medium led to a substantial improvement in the frequency of green plant regeneration. High concentrations (6-12%) of maltose gave greater number of green plants than low (1-3%) concentrations. In addition, plant regeneration was predominantly via an embryogenic route rather than an intermediate callus phase. Three commercially important spring barley cultivars, Tweed, Tyne and Natasha were considered. All three responded on a maltose-based medium, although to differing extents. The genetic stability of anther culture-derived lines produced by this method was assessed by karyotype analysis, and by using a range of biochemical and molecular markers. Low levels of variation were detected and the relative stability of the system may be attributed to the embryogenic mode of regeneration attained on a medium containing maltose. Despite the improvements obtained using the maltose protocol, response to anther culture was shown to be largely dependent on genotype. An investigation of the transmission of anther culture responsiveness into F1 hybrids produced from H.vulgare x H.spontaneum crosses showed that responsiveness was dominant to non-responsiveness. A high degree of heterosis for anther culture response was also observed in F1 progenies. An investigation of anther culture response in the wheat/barley disomic chromosome addition lines showed that addition of a single pair of barley chromosomes could not positively modify anther culture response in an unresponsive wheat background. The implications of these results are discussed in relation to the application of anther culture technology to barley breeding and genetics.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available