Use this URL to cite or link to this record in EThOS:
Title: A study of normal and abnormal forms of prion protein in the peripheral blood and tissues of patients with variant Creutzfeldt-Jakob disease
Author: Fagge, Timothy James
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 2005
Availability of Full Text:
Access from EThOS:
Full text unavailable from EThOS. Please try the link below.
Access from Institution:
This thesis assesses the potential use of PrPc as a surrogate marker for CJD by an analysis of blood from vCJD patients, sCJD patients, non-CJD neurological controls and healthy adults. PrPc was measured by DELFIA and cell-associated PrP was measured by flow cytometry. These are differences in free and cell-associated PrP found in blood of CJD patients and control groups, some of which may be useful as surrogate markers of disease DELFIA analysis identified a significant reduction in the concentration of PrPc in the whole blood of vCJD and non-CJD neurological patients compared with healthy adults. A significant elevation was found in plasma PrPc in sCJD patients compared with healthy adults and neurological controls. Flow cytometry found no significant differences between groups in the expression of PrP on platelets and lymphocytes. Neurological controls show significantly less PrP on red cells than healthy adults. In addition the development of a DELFIA based test designed for the detection of the disease-associated PrPSc in human peripheral blood is the other main focus of research studies detailed within this thesis. Sensitive assays have been developed using existing techniques allowing the detection of PrPSc in the central nervous system and peripheral lymophoreticular tissues of patients with vCJD as validatory studies prior to application of these assays to patient blood samples. Atomic dielectric resonance spectroscopy analysis techniques have also been used to investigate potential differences in frequency and atomic resonance, which may allow identification of characteristics distinct to vCJD peripheral blood samples.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available