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Title: Observations on the diagnosis of bovine virus diarrhoea in the live animal
Author: Elhassan, Awad Elkarim Mohammed
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1979
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Confirmation of a provisional diagnosis of bovine virus diarrhoea in the live animal is difficult. The best method proved to be the isolation of virus in cell cultures from nasal and faecal samples virus being sought after three blind passages either by its ability to interfere with a cytopathic strain of the virus or by identifying specific antigen by immunofluorescence. Reliability was only achieved when strict controls are applied at each stage; in particular cell cultures and sera had to be shown to be free of virus and/or antibody. For example 40 percent of the bovine embryonic kidneys collected for the preparation of cell cultures were found to be infected and most lots of foetal calf serum and all new born calf sera contained antibody. Cryopreservation of bovine embryonic kidney and calf testes cells with Dimethyl sulphoxide did not affect their viability or susceptibility to bovine virus diarrhoea virus while aliquots were screened for viruses. More samples gave virus in lamb testes cells, but the lamb testes cells deteriorated on storage. Further improvements were achieved when cells were propagated in antibody free serum which was inactivated at a temperature high enough to inactivate the virus without affecting the growth promoting activity of the serum and maintained in serum-free medium. The relationship of bovine virus diarrhoea virus and rubella virus was investigated. Rubella virus interfered with bovine virus diarrhoea virus in bovine embryonic kidney cells but there was no cross neutralization between them and bovine virus diarrhoea antibodies did not inhibit haemagglutination by rubella virus. All strains of bovine virus diarrhoea virus and border disease virus were so closely related that they were considered to be strains of the same virus.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available