Use this URL to cite or link to this record in EThOS:
Title: Allergic response to glomerular basement membrane in patients with glomerulonephritis
Author: Macanovic, Momir
Awarding Body: University of London
Current Institution: Imperial College London
Date of Award: 1973
Availability of Full Text:
Access from EThOS:
Access from Institution:
Glomeruli were isolated from normal human kidney cortex; glomeruli basement membrane prepared by ultrasonication of glomeruli, and soluble G.B.M. antigens finally prepared by proteolytic digestion of G.B.M. with collagenase. Specific anti-G.B.M. serum was produced in rabbits. The main antigenic components of soluble G.B.M. were immunochemically characterised as: one component of molecular weight over 200,000, the second component of molecular weight in the 20,000-100,000 and the third component containing small molecules of less than 10,000. Two high molecular components retained the antigenic properties, the last had been degraded to small non-antigenic fragments. Separate G.B.M. fractions are not uniform, but composed of multiple components of different molecular weight and size. In assessment of humoral allergic response to G.B.M. indirect immunofluorescence was found to correlate with the direct immunofluorescent method; double diffusion was found to be insufficient for the detection of circulating anti-G.B.M. antibodies; and among passive haemagglutination methods, glutaraldehyde method f chemical linkage of G.B.M. antigens to erythrocytes-was proved to be highly sensitive. Antibodies had been detected in rabbit anti-G.B.M. serum diluted more than 1:500,000. Circulating anti-G.B.M. antibodies were found in high titre in patients with linear deposits of IgG on the G.B.M. Patients with granular deposits of IgG had low titres of anti-G.B.M. antibodies. Predominantly IgG antibodies were eluted from kidneys of a patient with Goodpasture's syndrome and were shown to fix in vitro to homogenates of various organs, but predominantly to kidneys and lung. Evidence for cell-mediated allergic response to G.B.M. and streptococcal membrane antigens was found in patients with proliferative glomerulonephritis independently whether the immunofluorescent appearances suggested nephritis due to immune complexes or anti-G.B.M. antibodies.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available