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Title: The flexibility of the erythrocyte and the rate of sickling of cells containing haemoglobin
Author: Rampling, Michael William
Awarding Body: University of London
Current Institution: Imperial College London
Date of Award: 1972
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Investigations are reported of the effects on the sickling phenomenon of various physico-chemical factors, e.g. temperature and pH. These lead ultimately to measurements of the rate at which erythrocytes containing haemoglobin S will sickle after the rapid removal of oxygen by solutions containing sodium dithionite. Sickling has been shown to be a two-stage process. The first is a very rapid gelation of the haemoglobin, delayed by about 30 ms with respect to deoxygenation; this produced bizarre-shaped cells. The second is a much slower process, as a result of which the erythrocytes acquire the more normal shapes associated with the sickling phenomenon. A centrifuge technique for measuring erythrocyte flexibility has also been investigated and shown to give an index of this variable. The technique has been used to measure the effects of various anticoagulants on this flexibility. Heparin has been shown to be the best of the anticoagulants investigated for short term storage, while A.C.D. was the best for the long term. Low molecular weight dextran has been shown to induce inflexibility in erythrocytes and a mechanism for its action has been proposed.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available