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Title: T lymphocytes in intraocular inflammation
Author: Charteris, David Graham
Awarding Body: University of Edinburgh
Current Institution: University of Edinburgh
Date of Award: 1994
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Pathological and immunohistochemical analyses of two forms of human intraocular inflammatory disease, multifocal posterior uveitis and Behcet's disease, were carried out. Studies of post-mortem eyes from a patient with multifocal posterior uveitis revealed a focal lymphocytic infiltrate in the choroid, extending through Bruch's membrane to the retina. Endothelial cells of choroidal vessels at the inflammatory foci had the morphological appearance of "high endothelial venules" known to be involved in the selective trafficking of lymphocytes. Immunohistochemical analysis of the lymphocytic infiltrate showed that 70-80% were T cells. Immunohistochemical analysis of enucleated and post-mortem eyes from Behcet's disease patients showed that the mixed leukocytic infiltrate found in the retina and uveal tract was composed predominantly of T lymphocytes and macrophages. The retinal vessels were hyalinised and narrowed in the post-mortem material and were noted to have IL2 receptor positive T lymphocytes in their walls. Aberrant expression of MHC class II antigens by organ-resident cells in these studies was minimal. Experimental work on an animal model of intraocular inflammatory disease, experimental autoimmune uveoretinitis, was undertaken to determine the in vivo lymphokine production of T lymphocytes in inflammatory eye disease. The T lymphocyte infiltrate in active EAU was identified immunohistochemically as was interferon-γ protein and IL2 receptor positive cells. cDNA probes specific for rat interferon-γ, IL2, lymphotoxin and IL4 mRNA were amplified, extracted and radiolabelled. These probes were used to identify autoradiographically mRNA for these lymphokines in the destructive tissue pathology in EAU using the technique of in situ hybridisation.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available