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Title: UV-induced DNA damage and repair in microorganisms
Author: Morse, N. R.
Awarding Body: University of Wales Swansea
Current Institution: Swansea University
Date of Award: 2001
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The formation of Cyclobutane Pyrimidine Dimers by Ultraviolet irradiation is widely linked with the formation of skin cancer and their effects can be adequately seen in the symptoms of Xeroderma Pigmentosum patients. However, recently the germicidal effects of UV have been harnessed to disinfect wastewater: it produces the required bacterial and viral log kill. Presented here is a study developing a novel molecular assay that can specifically detect the damage produced during UV disinfection, and quantitatively analyse the amount of UV light absorbed by the final effluent bacteria. Standards were developed under specific manufacturers' conditions using a collimated beam apparatus. The immunoslotblot assay was tested using a controlled pilot plant system, which identified anomalies between the administered dose and the absorbed dose. An intrinsic variation experiment showed the assay worked efficiently. Subsequent analysis was performed on eight operational wastewater treatment plants situated around the coast of Wales. It was obvious that the administered UV dose, used to monitor the treatment plants, was not accurate as no correlations were observed with the log kill of the bacteria measured by six different procedures. Consequently, the immunoslotblot assay developed here revealed highly significant correlations with all log kill analyses, indicating that the substantive situation is being measured. UV-induced DNA damage and its subsequent repair was also studied at the level of the nucleotide in Saccharomyces cerevisiae. This identified an influence of nucleosomes in the repair rates of CPDs. This technique is too detailed and time-consuming to be used for routine water monitoring.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available