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Title: The toxicity and accumulation of heavy metals by species of marine phytoplankton in culture
Author: McGlone, G.
Awarding Body: University College of Swansea
Current Institution: Swansea University
Date of Award: 1979
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The toxicity of nickel to Phaeodactylum tricornutum has been estimated using 14C-fixation and growth curve techniques. The accumulation of nickel has been followed using 63N1. The binding of nickel was found to conform to saturation kinetics and binding constants have been determined. 10-3M EDIA was found to remove approximately 70% of the bound nickel. Experiments on the effect of temperature on nickel uptake, and the uptake of nickel by living and killed cells have supported the suggestion that nickel uptake is a passive process. A model for nickel binding, based on nickel efflux kinetics, has been proposed. The growth of Chlorella stigmatophora in medium containing nickel, cadmium or zinc has bean investigated. The accumulation of 63 Ni, 6'5Zn and 109Cd has been followed. Binding of these metals has been found to conform to saturation kinetics and binding constants have been estimated. EDTA was found to remove bound nickel and zinc from calls of C.stigmatophora, but to have no affect on bound cadmium. Zinc and cadmium were found to compete mutually for binding sites, but these metals did not compete with nickel and vice versa. No evidence has been presented for an active component of nickel, zinc or cadmium uptake. It has been suggested that the principal binding groups for zinc and cadmium in C.atigmatophora are sulphydryl.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available