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Title: Optimisation of high performance liquid chromatography
Author: McCann, M.
Awarding Body: University College of Swansea
Current Institution: Swansea University
Date of Award: 1983
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The theory of liquid adsorption chromatography is reviewed. Preliminary experiments involving the eluting of aromatic alcohols with mixtures of petroleum spirit (bp 30-40°C) and ethanol from silica were carried out and a new approach to the theory is developed. This is based on a Langmuir competitive adsorption model and leads to an equation linearly relating inverse net retention volume of the solute to volume fraction composition of the mobile phase. The theory was tested with experiments on systems in which solvent composition was varied over the entire volume fraction range. Results are included also for elution of a series of ketones by mixtures of hydrocarbons and tetrachloromethane from a reverse phase silica. Good agreement was obtained between the partition coefficients calculated from the results, predicted by the model and values deriving from direct partition experiments based upon gas chromatographic analyses. Further modifications to the theoretical model were then introduced to cover the characteristic behaviour of all the systems studied.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available