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Title: Studies of liquid chromatography
Author: Madden, S. J.
Awarding Body: University College of Swansea
Current Institution: Swansea University
Date of Award: 1983
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The first section of the thesis examines the problem of saccharide separation by H.P.L.C. with special reference to the speed as well as the ease of separation. Previous work is extensively reviewed and several types of common systems are studied with a view to elucidating the underlying problems of sugar separation. Inherent system inefficiency is identified as being responsible for the poor results obtained by earlier workers and confirmed here. An optimisation programme was developed to identify the best conditions for the best systems examined. Although the separations achieved are not comparable with those attainable with other sample types, they represent a substantial improvement so far as sugar analyses are concerned. The second section deals with developments in mixed-solvent theory for liquid chromatography. The theories developed over the past two decades are critically reviewed and tested with a variety of ternary systems over the complete range of binary solvent composition. It was found that the retention behaviour of the majority of systems could not be adequately explained by the theories so far developed. A comparison of the models showed the Langmuir/diachoric models which relate inverse corrected retention volume of the solute to volume fraction of the mobile phase to be the most suitable and further modifications to this model are made to encompass all of the systems studied. A general, semi-empirical equation has been found, that is capable of describing any of the six separate types-of inverse retention-volume fraction plot identified in this work. This is the first occasion on which the range of types of plot occurring has been established and also, the first, on which a satisfactory general equation has been identified.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available