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Title: Bacteria isolated from separated organs of Mytilus edulis collected from sewage polluted shores
Author: Al-Jebouri, M. M. H. M.
Awarding Body: University College of Swansea
Current Institution: Swansea University
Date of Award: 1980
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The effects of site and season on the numbers of bacteria from different organs of mussels, Mytilus edulis, were studied. These mussels were collected from four different shore sites at various distances from the urban effluents during the winter and during the summer. This study utilized several media (MacConKey agar. ZoBell medium, TCBS and BTBST media, and CLEO medium) and identification techniques (conventional and API20E system). Total coliforms at 250°C and at 37°C, viable counts of marine heterotrophs and Vibrio parahaemolyticus were obtained. CLEO medium gave an excellent growth and characteristic colony morphology of the following bacterial types: Escherichia coli, Shigella dysenteriae, Klebsiella pneumoniae, Enterobacter cloacae. Serratia sp., Yersinia enterocolitica, Erwinia herbicola, unidentified enterobacteria, Pseudomonas spp., Aeromonas hydrophila, Flavobacterium sp., Pasteurella sp., Acinetobacter calcoaceticus var., unidentified Gram-negative rods, Micrococcus spp., Staphylococcus spp., unidentified Gram-positive cocci. Bacillus spp., Corynebacterium sp., Kurthia sp., unidentified Gram-positive rods and unknown bacteria. The cleansing of mussels by UV-irradiation of the recirculating seawater was monitored by counting E. cola, coliforms at 25°C and at 37°C and marine heterotrophs: these four bacterial types were also investigated to study the effect of a rinsing treatment on the mussel organs by sterile seawater before dissection. The mussel organs (foot. labial palp, stomach, intestine, gill, mantle) and the residual tissues were investigated and the higher bacterial loads were from the digestive tract (intestine and stomach). The highest bacterial numbers and the greater variety were isolated from the highly polluted sites. The effect of season on bacteria isolated was significant and their higher numbers were usually in the winter.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available