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Title: Factors affecting the sensitivity to insulin of diaphragm and adipose tissue in obese mice and their lean littermates
Author: Abraham, Ralph Richard
Awarding Body: University of London
Current Institution: Imperial College London
Date of Award: 1971
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1. The sensitivity and response to insulin in vitro on (a) the incorporation of glucose-U-14C into glycogen in the isolated diaphragm muscle, and (b) the oxidation of glucose-1-14C in the epididymal fat pad and in adipocytes, has been studied in obese hyperglycaemic mice fed ad libitum (ob/ob), in obese mice maintained on a restricted diet (ob/ob-RD), and in lean mice, at 2-4 months of age and at 6-8 months of age. 2. In both age groups there was a significant, although reduced, response to insulin in the muscle from obese mice but this was normal in the 6-8 month old group of ob/ob-RD mice. 3. The glucose oxidation in the epididymal fat pad of the obese mice fed ad libitum was very reduced in both age groups but it was normal in fat pads from fed obese mice kept on a restricted diet. There was no insulin effect on the fat pad of obese mice in either age group. The response and sensitivity to insulin was partially restored in the ob/ob-RD mice. 4. There were only small differences in sensitivity between adipocytes prepared from obese and lean epididymal fat pads. 5. These results suggest that a decreased sensitivity to insulin at the cellular level is not the primary defect in the obese-hyperglycaemic syndrome. 6. When expressed per unit weight of tissue, there were fewer and larger adipocytes, with a smaller vascular space, a smaller water content and the same nitrogen content in pads from ob/ob mice compared to lean mice. 7. The plasma concentration of immunoreactive insulin, (IRI), in the fed or fasted state or after chronic restriction of food intake, was higher in obese than in lean mice. In response to glucose,the plasma IRI concentration 60-90 mins. later was higher in fed or fasted obese mice. 8. These results are discussed together with related findings in experimental and genetic obesity.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available