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Title: Analysis of novel mouse Y chromosome genes in differentiating germ cells
Author: Ferguson, L.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2009
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Oligonucleotide arrays identified a global up-regulation of X and Yp linked genes in mouse models with deletions of MSYq. In combination with previous cDNA array data, the oligonucleotide array data identified four genes with little or no previous characterisation. Two of these genes (h2aly and AK006152) were mapped to the short arm of the Y chromosome outside of the Sxrb interval. H2aly is part of a novel histone family, most closely related to the H2A.Bbd family and has multi X linked relatives and an autosomal relative. This protein coding family is expressed specifically in spermatids. No human ortholog was identified. AK006152 is a novel, mouse specific single or low copy Y linked gene. It has a potential open reading frame and is expressed specifically in spermatids. The two multi-copy genes (Orly and CJ175665) were significantly down-regulated in the mouse MSYq deletion models and subsequently mapped to the long arm of the Y chromosome. Orly is a chimeric locus composed of Ssty1, Asty and Sly and generates many variant transcripts that show a spermatid specific expression. No open reading frame was identified in Orly transcripts and anti-sense transcripts may be involved in the regulation of Sl. .CJ175665 is composed of exon 6 of Sly and the surrounding 3’ and 5’ intronic regions and was also expressed in spermatids only. An antibody raised against a peptide designed from the ORF of CJ175665 localised to the nucleus and produced a testis specific band in Western blotting. However, RT-PCR analysis detected other transcripts from the Sly locus containing intronic sequence which could not exclude the possibility of unprocessed transcripts. Anti-sense transcripts also detected from the Sly and CJ175665 loci also indicate a role for self-regulation of transcription.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available