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Title: Chromosome rearrangements in breast tumour cell lines
Author: Davidson, J. M.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2001
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This Thesis describes experiments carried out to identify the chromosome abnormalities, and complete karyotypes of a panel of breast cell lines by 24-colour fluorescence in situ hybridisation (FISH). 24-colour FISH allows all 24 human chromosomes to be identified simultaneously. Using 24 different combinations of up to five fluorochromes each chromosome is hybridised with a unique probe mixture. Computer software identifies wavelength differences between the different probe mixtures and colours each chromosome a different colour. In this way a translocation composed of three chromosomes will hybridise in three different colours, and the component chromosomes can be easily identified. The karyotypes of the breast cell lines were compared to each other, to previous G-banding karyotypes, and to fresh breast tumours. The cell lines fall into two main categories, those with near-diploid modal chromosome number and few rearrangements; and those with near-triploid modal chromosome number and many rearrangements. The 24-colour karyotypes can elucidate complex chromosome rearrangements intractable to G-banding analysis. This was clearly illustrated in several cell lines with complex co-amplification of two chromosomes, for example co-amplification of 8 and 11 in MDA-MB-134; and 8 and 17 in ZR-75-30. Also 24-colour FISH allows the elucidation of highly complex marker chromosomes in, for example, SK-BR-3, which involve six or more different chromosomes. The rearrangements identified in the breast tumour cell lines compared well with those identified in fresh breast tumours and in breast tumour cell lines by conventional cytogenetics and 24-colour FISH. Translocations of chromosomes 8;11 and 1;20 are equally as frequent in breast cell lines as in the primary tumours, and 8, 16 and 11 are the chromosomes most frequently involved in translocations in both the cell lines and the primary tumours.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available