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Title: Investigation of gene expression patterns in normal juvenile mouse testis and infertile mouse models
Author: Clemente, Emily
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2006
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This thesis details the use of a series of cDNA libraries corresponding to several testis cell-types derived from juvenile mouse testes to investigate (i) the first wave of spermatogenesis in the wild-type mouse and four germ cell depleted mouse models where mutations have abrogated spermatogenesis at different points in the pathway (ii) mouse mutants with progressive deletions on the Y chromosome that perturb spermiogenesis (the final maturation stages of spermatogenesis). The gene expression patterns generated were validated by extensive comparison to existing data in the literature and the use of real time reverse-transcription PCR and RNA in situ hybridisation (ISH). This analysis permitted (i) the establishment of thousands of gene expression patterns that were associated with the appearance of particular germ cell-types at different stages of the first wave; (ii) the classification of genes into groups associated with spermatogenic stages and somatic or germ cell lineages, this allowing key genes and molecular pathways active at different stages of the spermatogenic pathway to be identified; (iii) the identification from the study of the Yq deleted models, of new genes mapped to the Y chromosome and other candidate genes with a potential role in the infertility phenotypes associated with these Yq deletions. The study has identified differential gene expression of a large number of novel genes active in spermatogenesis. This underlines the potential of such genome based approaches to identify novel genes that will help to provide a better understanding of the genetic networks underlying germ cell development. The value of such a study lies in the scope to unravel causes of infertility and the contribution that this can make to the development of therapies.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available