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Title: Plastid protein synthesis and plastid-to-nucleus signalling
Author: Choy, M.-K.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2007
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Two plastid-to-nucleus signalling pathways had previously been identified by studies on genomes uncoupled (gun) mutants of Arabidopsis. Five putative gunl-like mutants from a new collection of Arabidopsis gun mutants with a green fluorescent protein (GFP) marker gene under the control of a tobacco RbcS (encoding ribulose-1,5-bisphosphate carboxylase small subunit) promoter were examined further. One of the mutant lines, PR48.2N, showed two-fold higher transcript abundance of nuclear photosynthesis genes, RBCS and LHCB1 (encoding light-harvesting chlorophyll a/b-binding protein 1), compared to wild type with or without treatments of norflurazon or lincomycin. Pigment analysis of PR48.2N seedlings, illuminated for 16 hours after being subjected to various lengths of dark treatment demonstrated that the mutant line accumulated less chlorophyll than wild type after short periods of darkness (2-4 days) but showed an enhanced ability to green after prolonged dark treatments (5-10 days). Consistent with the enhanced greening ability, transcript abundance of nuclear photosynthesis genes was higher and there was more thylakoids membrane in chloroplasts in greened PR48.2N seedlings after prolonged darkness compared to the wild type. Microarray analysis indicated that a group of transcripts encoding seed storage proteins, oleosins and late embryogenesis abundant proteins showed very low abundance in PR48.2N seedlings. The promoter regions of the genes shared some cis-elements possibly involved in regulation by ascisic acid (ABA). However, the ABA content of PR48.2N seedlings was not significantly different to wild type, although the germination of mutant seeds was more sensitive to inhibition by ABA than the wild type.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available