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Title: Human decidual NK cell receptors and their functions
Author: Chang, C.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2002
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In this thesis I have studied the possible receptors on decidual leukocytes which will bind to the HLA class I ligands on trophoblast. Of particular importance is the identity and function of the receptor for HLA-G as this molecule is only found on invading trophoblast cells. I have studied the expression, binding characteristics and functions of two possible HLA-G receptors, KIR2DL4 and ILT2. cDNA for these receptors was transfected into NK cell line, several functional assays were performed including cytotoxicity and cytokine production. The results showed the recognition of HLA-G by ILT2 and the functional consequences in these in vitro studies. There was no report on KIR2DL4 antigen expression in decidual NK cells. A rabbit polyclonal antibody against KIR2DL4 was made and purified. KIR2DL4 protein was detected from NK cell lysate in a Western blotting assay with this rabbit antibody. To further characterise the possible outcome of this maternal interaction in pregnancy, first I studied the cytokine profiles of decidual leukocytes, NK cells and trophoblast. In addition to previously published cytokines (GM-CSF and TNF-a), IL-6 and IFN-g have been found to be produced by decidual NK cells. These cytokines could have some major functions in the roles NK cells play in pregnancy. To dissect the change of cytokine profile after decidual leukocytes' recognition of placental cells, an in vitro culture system was developed using primary trophoblast cells and isolated decidual leukocytes. We found two cytokines (IFN-g and TNF-a) have been altered in this co-culture system. And this alteration could be due to the IL-10 and TGF-b1 produced by trophoblast. These results may shed some light on the mechanism and result of implantation.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available