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Title: The family 2 of G-protein coupled receptors (GPCRs) in two marine teleosts, Fugu rubripes and Sparus aurata
Author: Cardoso, J. C. R.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2003
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The family 2 of receptors belongs to the structurally conserved seven transmembrane receptor family of GPCRs (G-protein coupled receptors). This multigene family includes receptors for the physiologically important small hormones and/or neuropeptides such as VIP, PACAP, GHRH, CRF, secretin and calcitonin. The overall objective of this thesis was to identify and isolate family 2 members of the GPCR superfamily in the compact genome of the teleost fish, Fugu rubripes. Using heterologous probing of the Fugu cosmid library and data mining of the Fugu Genome Consortium database, 21 receptors of the family 2 of GPCRs were identified. Only 15 members of this family have been found in human and analysis has shown that the “extra” receptors in Fugu are duplicates, not novel family members. This thesis will describe, in depth, the genomic characterisation and expression patterns of the Fugu VIP/PACAP, CRF1 and GHRH receptors. This work describes for the first time the duplication of GPCR family 2 members in vertebrates. As a prelude to further work, beyond the scope of this thesis, two cDNA clones for duplicated PAC1 receptors were isolated and characterised from the more experimentally amenable teleost, the sea bream (Sparus aurata). Additional, alternative splice variants were identified in this species. Expression profiles of the duplicated receptors are discussed with reference to the mammalian orthologues and the current state of knowledge on the gene function of these family members. The results of comparative genomic, expression and phylogenetic analyses of the genes identified in the two fish are discussed within the context of theories about gene duplication and preservation of duplicated genes.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available