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Title: Hydrodynamics and adsorption within an expanded bed adsorption column
Author: Bruce, L. J.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2001
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In this thesis, relationships between the hydrodynamics and adsorption behaviour within EBA columns were examined and methods to improve EBA performance in terms of productivity, reproducibility and control were provided. The effect of column verticality was quantified and identified as a significant source of process variability. Column misalignment of only 0.15° off vertical resulted in a greater increase in liquid dispersion in 1 cm diameter columns compared to 5 cm diameter columns. Protein breakthrough curves for vertical and 0.15° off-vertical runs performed using a 5 cm diameter column were essentially indistinguishable, while applicability more protein could be applied to a vertical 1 cm diameter column compared to the same column misaligned by 0.15°. This degree of misalignment was not detectable by visual assessment of adsorbent particle movement within the column, therefore methods were developed to ensure column verticality for separations at the smaller scale. Equipment and methods for in-bed sampling of both liquid and solids were developed. A 5 cm diameter EBA column was modified to allow abstraction of samples from ports positioned along the columns wall. The modifications to the column did not significantly affect liquid dispersion or separation efficiency. It was demonstrated that on-line, in-bed sampling can provide higher control of a separation. The in-bed monitoring system was also used to study hydrodynamics within a vertical 5 cm diameter column with a perforated plate distributor, the same column aligned 0.15° off-vertical, and a 5 cm diameter column which employs a stirrer for flow distribution.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available