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Title: The activation phenotype of adult CNS stem cells in response to demyelinating injury
Author: Bruce, C. C.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2010
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In this thesis, I initially explored the technique of laser capture microdissection to isolate a homogeneous population of adult oligodendrocyte precursor cells (OPCs) from remyelinating lesions. The aim was to use a microarray to fully characterise the phenotype of the activated OPC. I then investigated whether OPCs express markers associated with neural stem cells upon activation. The results indicated that activated OPCs can exhibit the characteristics of neural stem cells and suggested that an additional source of CNS stem cell does not contribute to the pool of remyelinating OPCs. Chapter 5 asked whether activation of OPCs results in reprogramming to a more pluripotent state. The results suggested that CNS demyelation does not induce reprogramming of OPCs. However, an Oct4 pseudogene was identified as selectively transcribed during the activation phase of remyelination. This highlights the caution that must be exercised when looking for the presence of embryonic markers in adult tissue. Chapter 6 investigated whether OPCs express markers associated with the embryonic neural crest as they are activated in response to CNS demyelination. The results suggested that although several genes associated with neural crest induction are expressed during remyelination, this is not followed by protein expression. This study provides in vivo evidence that adult OPCs exhibit more characteristics of multipotent stem cells than previously appreciated. These properties are unveiled by the activation of OPCs as they respond to CNS injury, indicating that demyelination may create an environment permissive to multi-lineage differentiation.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available