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Title: The quartz crystal microbalance as a tool to study orthopaedic biomaterials
Author: Booth, J. C.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2007
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This work aims to develop the quartz crystal microbalance with dissipation (QCM-D) to study cell adhesion. Firstly, several aspects of a commercial QCM-D were investigated and protocols developed to enable experimentation with protein adsorption to be carried out. Analysis performed with the frequency and dissipation responses obtained with the QCM-D during protein adsorption experiments enabled factors such as the degree of protein coverage, the thickness of the attached protein layer, and the dominant kinetics to be deduced from the Sauerbrey, Maxwell and Voigt models.  It was also possible to differentiate between bovine serum albumin and fibronectin proteins, and to confirm that a monolayer of each protein is formed on gold. The second part of this thesis considers cell adhesion. A protocol was developed using an axial flow chamber in a batch mode system that was able to monitor cell adhesion on gold and titanium surfaces. 3T3 fibroblast cell adhesion was found to occur more readily on titanium than gold, but adhesion could be enhanced on gold by pre-coating the surface with fibronectin. The Sauerbrey, Maxwell and Voigt models were found to be not meaningful for cell adhesion. Since information could not be extracted using these models, laser scanning confocal microscopy studies were performed in addition to the QCM-D experiments. The QCM-D signal produced during cell adhesion is dominated by the proteins present in the system. It was not possible to differentiate between fibroblast-like 3T3 cells and osteoblast-like MG63 cells. Some of the cell adhesion conclusions are preliminary, but the techniques required to perform such experiments have clearly demonstrated.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available