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Title: Characterisation of cholinergic interneurones in the rat striatum
Author: Bell, M. I.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2000
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The physiology and pharmacology of rat striatal cholinergic interneurones has been characterised using the whole-cell patch-clamp technique, in situ hybridisation, single cell RT-PCR and dual fluorescence immunocytochemistry. Cholinergic interneurones displayed a characteristic resting electrophysiology. The resting membrane potential was relatively depolarised and was associated with a relatively large input resistance. All neurones showed a characteristic reduction in their voltage-current relationship that corresponded to the hyperpolarisation-activated current (Ih). Action potentials were associated with a relatively long afterhyperpolarisation. Substance P caused a depolarisation in cholinergic interneurones via a NK1 receptor-mediated Ca2+- activated inward current. The inward current was inhibited by the phospholipase C inhibitor U-73122, and by the inclusion of the inositol 1,4,5 triphosphate receptor antagonist heparin in the electrode solution. These findings correlated with gene expression studies showing the presence of NK1 receptors in these cells. The non-selective metabotropic glutamate (mGlu) receptor agonist 1S,3R-APCD caused a depolarisation of interneurones via a group I mGlu receptor-mediated Ca2+-activated inward current. The inward current was carried by two ionic components and could be inhibited by the PLC inhibitor U73122 and the PKC inhibitor chelerythrine. These findings were consistent with gene expression studies showing the presence of mGluR1 and mGluR5 receptors in these cells. In addition, cholinergic interneurones expressed mGluR2 and mGluR7. Intrastriatal stimulation evoked fast synaptic currents that were mediated by NMDA, AMPA and GABAA receptors.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available