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Title: Characterisation of a guard cell GAL4 GFP enhancer trap population
Author: Baker, A.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2004
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Stomatal guard cells delimit the pores present in the leaves of all vascular plants, and organs from more primitive plants such as liverworts and mosses. Stomatal pores are the primary route for gas exchange between plants and the atmosphere. Guard cells control the aperture of the pore and their primary function is to optimise carbon dioxide uptake for photosynthesis and minimise transpiritional water loss. Guard cells have been studied extensively using a wide range of physiological techniques but current understanding of the molecular biology of guard cell function and development is less advanced. The goal of this project was to identify genes expressed preferentially in guard cells, and DNA sequences involved in the regulation of guard cell gene expression, using Arabidopsis thaliana GAL4 green fluorescent protein (GFP) enhancer trap lines. The GAL4 GFP enhancer trap involves GAL4-mediated activation of GFP expression after random integration of a T-DNA construct into the plant genome. The GFP expression patterns can be visualised using fluorescence microscopy and will most likely report the activity of a plant enhancer element in the vicinity of the T-DNA. In this project the GFP expression patterns and numbers of T-DNA inserts in a guard cell GAL4 GFP enhancer trap population were characterised. A detailed molecular analysis of four guard cell GAL4 GFP enhancer trap lines with single T-DNA inserts was performed. The position of the T-DNA inserts in the four single insert lines were determined and the expression patterns of the genes flanking the T-DNA inserts were characterised. The genomic DNA flanking the T-DNA in three of the single insert lines was also analysed, to identify regulatory DNA sequences potentially important in controlling guard cell gene expression.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available