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Title: Characterisation of 12 strains of Rhodococcus and their 3-(2-hydroxyphenyl) propionic acid (OHP) degradation pathways
Author: Andrews, J.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2002
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In this work a series of 11 Rhodococcus strains which mineralise the alkylphenone, 3-(2-hydroxyphenyl) propionic acid (OHP) were isolated from a larger strain collection previously isolated from a polluted gasworks in Newcastle, UK. These OHP catabolic pathways had not been subjected to direct selection during strain isolation and therefore are likely to represent the 'native' state of genes within the Rhodococcus genome. To investigate the Rhodococcus population structure, a phylogenetic analysis of the 11 ohp isolates of Rhodococcus and Rhodococcus sp. V49 from pristine soil was made and a biochemical characterisation of their OHP pathways was carried out. The results show a range in the mode of expression of analogous/homologous gene clusters in this group of closely related soil bacteria. The taxonomic analysis demonstrates that of the strains isolated from a polluted gasworks in Newcastle, all 11 are independent isolates of Rhodococcus, including the species R. ruber, R. erythropolis and R. opacus, although they originate from a narrow taxonomic radius. Rhodococcus sp. strain V49 which was isolated from pristine soil in Canada, is also a member of this phylogenetic group which encompasses the 13 described species of Rhodococcus. Rhodococcus sp. V49 is used as a model system in this work for examining ohp catabolic genes, as it has been the subject of previous work. These data argue that the soil environment is finely divided and supports a diverse array of closely related but distinct species from numerous clones. Rhodococcus strain RC291 appeared to exhibit catabolite repression during the initial characterisation of the OHP catabolic pathways.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available