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Title: Astrocytic inhibition of neurite outgrowth at the Schwann cell/astrocyte interface
Author: Adcock, K. H.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 2000
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The objectives of this thesis are to create a simplified in vitro model that mimics the neurite behaviour at the interface between the CNS and PNS and to overcome the repulsion by astrocytes. This has been achieved by co-culturing astrocytes and Schwann cells and monitoring neurite behaviour on them. In such a model neurites extending from a dorsal root ganglion explant exhibit a preference for Schwann cells and rarely cross the interface to grow onto astrocytes demonstrating that there is similarly between the model and the in vivo situation. Various molecules have been implicated in the guidance of neurite trajectories and several classes of these have been investigated in the course of this thesis. Two classes of molecules have been identified as having a role in guiding neurites at the Schwann cell/astrocyte interface: the first a cell adhesion molecule, L1, the second requires intracellular signalling events to be triggered by a neurotrophin and cyclic AMP. The production and regulation of a class of protease inhibitors, the TIMP family, by astrocytes in vivo and in vitro was also investigated. Astrocytes make at least 3 of these proteins which could also contribute to the general lack of neurite outgrowth seen on astrocytes when there is a choice between Schwann cells and astrocytes.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available