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Title: Spindle pole body duplication in the budding yeast Saccharomyces cerevisiae
Author: Adams, I.
Awarding Body: University of Cambridge
Current Institution: University of Cambridge
Date of Award: 1999
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A microtubule-organising centre (MTOC) at each spindle pole is responsible for assembling the mitotic spindle. This thesis describes a molecular characterisation of MTOC duplication in the model eukaryote Saccharomyces cerevisiae. The SPB core, encompassing the central plaque and parts of the outer and inner plaques, is composed of only six main proteins. I have examined the arrangement of these molecules in the SPB, core, and their contribution to SPB structure. Both the duplication plaque and the satellite appear to contain the SPB core components Nudlp, Cnm67p, Spc42p and Spc29p, but probably not the nuclear SPB core components Spc110p-Cmd1p. Furthermore, although small haploid satellites have an indistinct morphology, larger tetraploid satellites are plaque-like. Thus the satellite and duplication plaque contain the same SPB core components and have similar morphologies. Spc110p appears to assemble onto the duplication plaque once it has been inserted into the nuclear envelope. Cytoplasmic mislocalisation of Spc110p disrupts the spatial and temporal regulation of SPB assembly producing an aberrant cytoplasmic plaque tethered to an attachment site at the distal end of the half-bridge. This attachment site presumably binds to central plaque components such as Spc42p and/or Spc29p to direct satellite assembly to the distal end of the half-bridge, and persists throughout SPB duplication to tether the central plaque to the half-bridge in fully-assembled SPBs. Thus I have shown that most of the main SPB core components are pre-assembled into a cytoplasmic plaque-like structure prior to SPB duplication. Self-seeded assembly of these components during SPB duplication may enlarge the satellite to form a duplication plaque that is then inserted into the nuclear envelope to allow assembly of nuclear SPB compounds.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available