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Title: Characterising the effects of phosphorus (P) supply on zinc (Zn) nutrition in Arabidopsis thaliana
Author: Alamer, Khalid Hasan
Awarding Body: University of Nottingham
Current Institution: University of Nottingham
Date of Award: 2013
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Phosphorus (P) and Zinc (Zo) are essential nutrients for plant growth. Plants typically have a [P] leaf of 3 mg P g-1 dry weight. Most plants contain leaf Zn concentrations [Zn]leaf of <0.1 mg Zn g- 1 dry weight (DW)- [P]leaf and [Zn]leaf vary substantially according to the characteristics of the growing media and due to genetic factors. A complex relationship between [P]leaf and [Zn]leaf, known as P-induced Zn deficiency (PIZnD), has been documented in several crop species, in which high levels of [p]ext appear to induce Zn deficiency symptoms, although the mechanism by which this occurs is not known. The aim of this project was to gain a greater understanding of the relationship between [P]leaf and [Zn]leaf in planta, using Arabidopsis thaliana (L.) Heynh as a model system. The first scientific objective was to explore the relationship between [P]leaf and [Zn]leaf at a physiological level, in which the growth and global mineral composition of leaves were determined over at a range of external concentrations of P ([P]ext). The second scientific objective was to determine the effect of [P]ext on global changes in gene expression. The overarching hypothesis was that increases in [P]ext lead to increases in [P]leaf which, in turn lead to down regulation of genes which are involved in Zn uptake and transport. Two sets of technical experiments were conducted to optimise an assay system used to induce P toxicity symptoms. First, to optimise leaf mineral analysis, plant certified reference materials were used to determine the minimum weight of material required for analysis using inductively coupled plasma atomic emission spectrometry (ICP-AES), and this was compared to established inductively coupled plasma mass spectrometry (ICP-MS) techniques. This experiment demonstrated that 0.3 g DW of a cabbage standard reference material was sufficient to accurately and precisely detect the composition of nine elements, including P and Zn. Second, plants were grown in an in vitro cultivation system comprising a minimal agar, supplemented with mineral salts at eight [P]"" levels (0 to 10 mM)- Shoot and root fresh and dry weights and mineral composition were measured after 14 d of growth.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available