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Title: Chemical and nutritional evaluation of Lupinus angustifolius L. (sweet lupin) seed proteins and its fractions on general metabolism of monogastric animals
Author: Rahman, Mohammed Habibur
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 1993
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The use of lupin seed, although a rich source of proteins, has been limited by the presence of toxic alkaloids. Recently a sweet, non toxic variety (it Lupinus angustifolius L.) has been developed in Western Australia, and reported to be safe for human consumption. However growth depression and low net protein utilisation (NPU) values in weanling rats fed raw L. angustifolius seeds have been reported in the literature. Thus seeds were analysed, and experiments were carried out with rats to evaluate NPU values and growth. Results indicated that L. angustifolius contains a far less reactive lectin than kidney bean, but that food intake and growth were depressed, due in part to essential amino acids (EAA) deficiencies. Supplementation with EAA improved growth, nitrogen retention and protein utilisation. However, when compared with that of the net protein utilisation values from rats fed on lactalbumin, the raw lupin seed meal was still found to be inferior, even when supplemented with EAA. Seed meal was fractionated into six components by sequential extraction with cold water, McIlvaine's buffer at pH 7.0, and dialysis of the supernatant. Analyses were carried out on lupin seed meal and fractions for carbohydrates, amino acids, oligosaccharides, minerals, phytates and run on SDS/PAGE to ensure consistency in fractions pooled for nitrogen balance experiments. It was revealed that a protein fraction resembling the gamma-conglutin, the simplest of the three globulins from the seeds of L.angustifolius, was extracted in almost pure form. A series of experiments was carried out to study the biological effects of all six fractions in growing rats. Results showed that seed meal and its fractions are unique in causing deleterious effects on liver, kidneys, spleen, thymus, heart, adrenal, stomach, caecum and colon but had no effects on pancreas.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available