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Title: Activation antigens on human glomerular mesangial cells
Author: Patterson, Angela M.
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 1999
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In the work described in this thesis the expression of activation antigens on human glomerular mesangial cells was investigated by selecting and subcloning hybridomas which secrete putative antibodies towards activated human mesangial cells. The panel of twelve monoclonal antibodies, produced in this way, was characterised to define the target antigens, cytokine receptors or cell adhesion molecules expressed as a result of mesangial cell activation. The twelve monoclonal antibodies were tested against non - stimulated mesangial cells and those stimulated with the growth factors PDGF or TGFβ1. Stimulation of the mesangial cells with PDGF or TGFβ1 was associated with an increase in the activity of the antibodies from tolerised mice. Comparing this significant difference in reactivity by these antibodies against non - stimulated and stimulated mesangial cells indicated that the antibodies were directed towards antigens expressed by stimulated mesangial cells. These antigens may be up - regulated antigens weakly expressed on non - stimulated mesangial cells or activation antigens expressed de novo. The reactivity of antibodies from the mouse immunised with PDGF stimulated mesangial cells against non - stimulated and PDGF stimulated mesangial cells was similar, indicating that the antibodies were directed towards cell surface antigens that are expressed to a similar degree by both non - stimulated and stimulated mesangial cells, that is, native antigens. The pattern of reactivity of antibodies from the mouse immunised with TGFβ1 stimulated mesangial cells, against non - stimulated and TGFβ1 stimulated mesangial cells, were similar to the reactivity shown by the antibodies from the corresponding tolerised mouse, indicating that the technique of tolerisation did not further enhance the specificity of the antibodies towards activation antigens. Seven antibodies of the panel of twelve monoclonal antibodies successfully detected antigens in normal and disease kidney. A striking pattern of reactivity against normal and diseased kidney was observed with three antibodies.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available