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Title: Digital imaging, leucocytes, gamma-linolenic acid and diabetic retinopathy
Author: Olson, J. A.
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 2000
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The work for this thesis has been carried out in the Diabetic Clinic, Woolmanhill, the Eye Clinic, Foresterhill and the Departments of Bio-Medical Physics and Ophthalmology, University of Aberdeen. One hundred and twenty two patients with type 1 diabetes mellitus were recruited from the Diabetic Clinic, Woolmanhill, and attended a Research Clinic at the Eye Clinic, Foresterhill, on a three monthly basis for two years each between 1992 and 1995. Data was extensively collated relating to diabetic control and diabetic complications. Concentrations and activities of several serum factors were studied in an attempt to elucidate pathogenetic mechanisms in diabetic retinopathy. Serum levels of soluble leucocyte endothelial adhesion molecules were measured to see if there is indirect evidence for increase leucocyte adhesion in diabetic retinopathy. Similar studies were also performed looking at serum induced retinal endothelial capillary cell migration, a putative early feature in the development of sight-threatening new vessel formation. Computer image analysis methods, developed in the Department of Bio-Medical Physics, were modified and evaluated, leading to robust techniques suitable for quantifying lesions of diabetic retinopathy in large numbers of unselected fundal photographs and fluorescein angiograms. This latter work was completed in 1997. As it is known that essential fatty acid metabolism in diabetes is abnormal, leading to low levels of gamma-linolenic acid and its metabolites, the above techniques were used to assess the progress of diabetic retinopathy in a two year crossover trial of dietary gamma-linolenic acid supplementation.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (M.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available