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Title: Physiological control of gene expression in adipose tissues of genetically obese (ob/ob) mice
Author: Nestor, Antoinette
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 1994
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Fatty acids and glucose are fuels of major importance to mammalian tissues, and their uptake is controlled by two key proteins, lipoprotein lipase (LPL), and in insulin-sensitive tissues, the glucose transporter GLUT4. This thesis describes the investigation of these proteins at the level of the gene, in brown adipose tissue (BAT), white adipose tissue (WAT) and heart of lean and genetically obese (ob/ob) mice. In the first physiological study, energy expenditure was increased by acute exposure to cold, and it was found that in lean mice this resulted in an increase in LPL mRNA levels in BAT and heart but there was a decrease in WAT. In obese mice, in contrast to the lean, there was no cold-induced incease in LPL mRNA in BAT or heart. There was an increase in uncoupling protein (UCP) mRNA levels in BAT of both lean and obese mice, although this increase was not as great in the obese. There was no effect of cold on GLUT4 mRNA levels in any of the tissues studied. The role of the sympathetic nervous system in the cold-induced changes in PLP mRNA was then examined, by administering noradrenaline. LPL mRNA levels increased in BAT of lean mice, but there was no increase in the obese animals. Noradrenaline appeared to have no stimulatory effect on LPL mRNA levels in WAT or heart of lean animals. The effect of the change in diet which occurs during the suckling/weaning transition were then investigated. Weaning resulted in an increase in LPL mRNA levels in BAT of lean animals, but not in the obese. There was no major effect of weaning on LPL or GLUT4 mRNA levels in the other tissues studied.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available