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Title: A study to investigate the effect of NO on myogenic development
Author: Mollan, Robin J. S.
ISNI:       0000 0001 3415 4436
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 1997
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The aim of the experiments presented in this Thesis was to determine if nitric oxide (NO) could modulate in vitro myogenesis since activity and immunolocalisation of the neuronal isoform of the enzyme, nitric oxide synthase was demonstrated in neonatal rat skeletal muscle. Therefore, it appeared possible that NO may influence the development of neonatal skeletal muscle. The NO donator, sodium nitroprusside (SNP), was used to determine the effects of NO on proliferation, fusion and myotube development in primary neonatal rat cultures and the mouse skeletal muscle cell line, C2C12. SNP treatment led to inhibition of fusion in both C2C12 and primary rat myoblast cultures. In addition, SNP reduced DNA synthesis in proliferating C2C12 cells and led to disintegration of rat primary myotubes. These effects of SNP were attenuated by preaddition of the guanylate cyclase inhibitor, methylene blue (MB) suggesting that NO release from SNP was influencing myogenesis via a cGMP-dependent pathway. In contrast, addition of 8-bromo-cGMP, a cell permeable analogue of cGMP was unable to mimic the effect of SNP treatment indicating that cGMP was not involved in the SNP response. Further investigation using oxyhamemoglobin as chelator of free NO did not reverse the effects of SNP Also, the effect of photolysed SNP on myogenesis was identical to that of freshly prepared SNP. These findings together suggested that the action of SNP was not via NO but was possibly an artifactual effect of metabolite(s) of SNP. Further investigation indicated that SNP treatment was toxic to myogenic cells, possibly via production of cyanide. In addition, the observation that MB could attenuate the toxic effects of SNP suggests that there is an interaction between these two compounds which is not dependent on NO activating guanylate cyclase.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available