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Title: Growth and differentiation of Mucor mucedo
Author: Leith, W. H.
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 1981
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1 Methods have been devised for growing mated and unmated surface cultures of Mucor mucedo to allow analysis of cell constituents during growth and reproduction. 2 Several strain differences were revealed. The minus strain has a 20% faster linear growth rate than the plus strain. The plus strain accumulated glycogen to a greater extent than minus. Measurements of the protein, ergosterol and β-carotene contents of the minus strain always exceeded those for the plus strain. 3 When two compatible mating types meet 'head on', the levels of protein and glycogen increases as the sexual hyphae, the zygophores, are induced, but these levels drop in the later stages of zygospore formation. 4 Very large accumulations of the isoprenoids ergosterol and β-carotene result when the two strains are mated or when they are treated with externally applied trisporic acid. 5 Methods were devised to study the growth enzyme, chitin synthase, from cell-free preparations and 'in situ' preparations of Mucor mucedo during growth and differentiation. 6 Chitin synthase was most active at the hyphal apices and decreased with age and differentiation. 7 Chitin synthase from Mucor mucedo has a pH optima of pH 5.5-6.0, temperature optima of 15-20°C, requires the Mg2+ ion and N-acetylglucosamine for activation and is inhibited by Polyoxin D and colloidal chitin. 8 A membrane-bound chitinase enzyme was extracted from surface agar grown cultures of Mucor mucedo. 9 The 3,4-dinitrophenyl-tetra-N-acetyl-β-D-chitotetraoside colorimetric test enabled a detailed study of chitinase. 10 Chitinase from Mucor mucedo has a pH 5.5 optima, a temperature optima of 55°C, does not require any metal cation for activation and is inhibited by colloidal chitin.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available