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Title: Electron spin resonance spectroscopy of biological materials
Author: Kent, M.
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 1967
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The work described in this thesis may be divided into three major sections. Firstly some of the techniques by which aqueous samples in particular whole tissue samples, may be studied using ESR are discussed. The long adopted procedure of employing flat cells in rectangular cavities to reduce the volume of sample in the region of microwave electric field is discussed in some detail. The limitations of this technique are discussed and the theoretical work of some workers on this topic is extended to include errors of placement of the sample. Brief mention is made of the way in which the maximum signal- to-noise ratio was sought in the design of a laboratory spectrometer with some emphasis on the use of multichannel analysers for this purpose since the laboratory built spectrometer described here was the first in this country to adopt such a system. Secondly, the experimental work performed on this and a commercially built spectrometer is described in great detail with respect to the results. All this work involves whole animal tissues, both rat and human. The results are obtained over a range of temperatures from 77 K up to room temperature. The low temperature results because of their greater complexity and detail tend to provide more information. The third section of this thesis is one which involves the explanation of many of the results found in the work. A detailed analysis of the effects of power saturation on spectra which are composed of unresolved hyperfine structure is carried out and the results of this section are used later to explain the experimental behaviour of the samples studied. It is found that the important result is that line widths which are unresolved can be estimated from the manner of saturation of the sample. This makes relaxation times accessible for study which would not be otherwise. Using these results many physical measurements are made on the ESR signals which many workers have assured could yield no information other than concentration of radicals.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available