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Title: Antigen presentation and Goodpasture's disease
Author: Jones, Victoria-Lynn
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 1998
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Goodpasture's, or anti-glomerular basement membrane (anti-GBM), disease is an uncommon, yet usually severe human autoimmune disorder characterised primarily by rapidly progressive glomerulonephritis (RPGN) and lung haemorrhage. The disease results from a sustained autoimmune response in which pathogenic autoantibodies are directed against the Goodpasture antigen [3(IV)NCI], a specific component, of certain basement membranes. Antigen presenting cells (APC) play a leading part in determining an individual's immune responsiveness by controlling how antigens will be displayed to CD4+ T-cells. APC are responsible for the internalisation, processing and presentation of extracellular antigens in association with MHC class II molecules at their surface. Further, the striking association between particular autoimmune diseases and HLA type suggests that HLA determined differences in presentation of self antigens may also influence the susceptibility to autoimmunity. The primary purpose of this project was to examine the Goodpasture antigen derived peptides processed and presented to T-cells by EBV-transformed human B-cells. This was achieved by following a sub-cloning strategy, in which cDNA from [3(IV)NCI], was stably transfected into a suitable cell line. A further batch of cells were maintained in identical conditions as a control. Following affinity purification, successive rounds of HPLC and mass spectrometry were utilised in order to identify putative antigen derived peptides. In addition to this, an immunoassay was developed in order to examine whether different affinity purified HLA-DR molecules (positively/negatively associated with Goodpasture's disease) bound synthetic Goodpasture peptides differently.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available