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Title: The role of the Ku complex in telomere replication timing
Author: Robertson, E. D.
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 2007
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In this thesis I examine three possible mechanisms by which the Ku complex may control the replication time of telomeric replication origins. Firstly I show that the relocalisation of an early origin to the periphery is not sufficient to alter its replication time and induce it to fire later in S phase proving that nuclear positioning is not sufficient for late origin initiation. Secondly I demonstrate that there is no increase in the general acetylation of histone H4 or histone H3 lysine 18 in the Δyku70 mutant at replication origins known to alter their replication timing programme in the absence of Ku, suggesting that chromatin modification is not the mechanism by which Ku specifies late telomeric origin firing. Finally, I show that Ku does not bind directly to telomere-proximal replication origins in vivo in yeast nor is it found to be present significantly far from the telomere end. In addition, I demonstrate that the Ku complex does not require the Ctf18-RLC complex to load it onto telomere ends.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available