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Title: Functional analysis of the bacterial CIC-type C1-channels/transporters
Author: Murray, E.
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 2007
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CIC-type C1- channels were recently identified in bacteria. The crystal structure of the E. coli C1C-type C1- channel C1cA was recently solved (Dutzler et al., 2002). However, the function of C1- channels in bacterial physiology has somewhat neglected. C1- ions are not thought to play a significant physiological role in E. coli. It was shown that an E. coli ClcA/C1cB double knockout mutant was more sensitive to an acid challenge in the presence of exogenous glutamate or arginine (Iyer et al., 2002). The overall objective of this study was to examine the physiological role played by the bacterial C1- channels during both extreme acid and growth permissive conditions. Studies have investigated the role of the channels in survival of extreme acid stress. The studies show that the activity of the channels sensitizes E. coli cells to extreme acid in a chloride-dependent manner. The analysis of the channels was extended through investigation of the properties of specific mutant channels that are expected to be affected in the gating region. These studies show that modifying the gate residues alters the impact of the C1cA protein on survival. Finally, studies undertaken for this thesis have investigated the expression of the C1cB channel and its potential contribution to acid sensitization at low pH.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available