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Title: Fabrication of molecular devices based on DNA self-assembly
Author: Szymonik, Michal Piotr
Awarding Body: University of Leeds
Current Institution: University of Leeds
Date of Award: 2012
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Advances in molecular engineering have enabled the formation of increasingly sophisticated molecular systems. Use of DNA and other biomolecules has proven a particularly powerful tool for nanotechnology, with their unique chemistry allowing the synthesis of self-assembling nanoscale devices with complex structures and functionalities. The ability to integrate such constructs with solid state electronic devices would be of great value for the development of these technologies into practical devices. In this project, a method was developed allowing the specific targeted alignment and binding of single molecules to sites on nano-patterned metal electrodes, relying on the highly specific molecular recognition capabilities of DNA. The patterning method utilised self-assembled monolayers of I-mercapto11- undecanol as a molecular resist, which could be removed via reductive electrochemical desorption of the gold-thiol bond. This allowed the patterning of thiolated DNA probes on selected electrodes in an array. A DNA strand with sticky ends complementary to the surface probes can then specifically bind to the surface, bridging between sites where this enables the simultaneous hybridisation of both its single stranded regions. The surface binding and hybridisation of thiolated DNA oligonucleotides was tested using a colorimetric surface staining technique and the quality of monolayers was investigated using several methods. These trials informed the development of DNA-coated surfaces resistant to non-specific binding. The electrochemical desorption of SAMs was then investigated as a means for the high-resolution patterning of surfaces. Employing these techniques, the specific bridging of gold electrodes separated. by 70nm with 330 basepair DNA strands was demonstrated. Additionally, the selective thermal melt ing of different DNA probes and the ligation of surface-bound DNA constructs were examined as further methods of controlling the specificity of the assembly reaction.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available