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Title: The role of asprin in phosphate buffered sucrose solution
Author: McKenzie, Shireen Natasha
Awarding Body: University of Leeds
Current Institution: University of Leeds
Date of Award: 2010
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Ischaemia-reperfusion injury still remains a problem in static hypothermic perfusion of organs for preservation. Many alternatives forthis are now being developed, such as continuous normothermic perfusion, however these methods are more costly and not as practical. Phosphate buffered sucrose (PBSL) solution has undergone extensive development and experimentation and has now reached the final stage. The isolated reperfusion liver model, which has been validated within our laboratory, was once again used to firstly determine whether aspirin had a role in PBSL and secondly whether it attenuated any of the ischaemia- reperfusion response. The 0.38 mole dose of Aspirin was then substituted for Piracetam, a more specific thromboxane A2 inhibitor , to elucidate if there were any additional benefits. The bile flow rate, liver enzymes, weight change, oxygen consumption and histology were used as parameters for analysis. The novel part of this work is the use of histology for comparative analysis. This assists in the understanding of what is occurring biochemically. The initial histological analysis on this work was H&E, but this was extended in later work on the same samples, to PAS and Gordon and Sweet. The histology of this later work can be found in appendix I. This was also later published. The resulting best solution was used on marginal porcine donors as a test of the robustness of the solution.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available