Use this URL to cite or link to this record in EThOS:
Title: Interspecific interactions between saprotrophic basidiomycetes : effect on volatile production and gene expression of mycelia
Author: Eyre, Catherine
ISNI:       0000 0004 2750 3249
Awarding Body: Cardiff University
Current Institution: Cardiff University
Date of Award: 2007
Availability of Full Text:
Access from EThOS:
Access from Institution:
Saprotrophic basidiomycetes play key roles in decomposition and nutrient cycling within woodland ecosystems. Species compete for space and resources, resulting in interactions with a range of outcomes, ranging from deadlock to replacement. Trametes versicolor was chosen to study these interactions in more detail and at a molecular level. During interactions T. versicolor produced barrages of aerial mycelium at the interaction front, and hyphal growth was inhibited in the presence of an opponent prior to contact. Volatile sesquiterpenes and aromatic hydrocarbons were produced when T. versicolor interacted with Stereum gauspatum, which may have inhibitory effects and cause DNA and protein damage. Suppression subtractive hybridisation libraries were constructed for the interaction of T. versicolor vs S. gausapatum. This is one of the first studies to examine interspecific interactions of saprotrophic basidiomycetes from a molecular perspective. Expressed sequence tag analysis coupled with cDNA microarray technology was used to study the molecular basis of interactions of T. versicolor with S. gausapatum, Bjerkandera adusta and Hypholoma fasciculare, which are replaced, deadlock and replace T. versicolor, respectively. Analysis revealed up-regulation of peroxidases, catalase, chaperone proteins and fungal cell wall enzymes, common to interactions. These genes may be employed to deal with an oxidative environment and intracellular damage generated during interactions and responsible for changes in morphology. More genes were common to interactions in which T. versicolor deadlocked with, or replaced its competitor, than when it was replaced itself. Different mechanisms may be employed against different species resulting in the range of outcomes observed.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available