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Title: Biochemistry of transgenic wheats
Author: Beacham, Tracey
ISNI:       0000 0004 2748 923X
Awarding Body: Cardiff University
Current Institution: Cardiff University
Date of Award: 2006
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This investigation uses transgenic wheat lines carrying the Pisum sativum glycerol 3-phosphate acyl transferase gene and /or the Arabidopsis thaliana acyl ACP thioesterase gene to determine their role of these genes in producing the altered metabolic and physiological traits seen in wild type plants grown under enhanced greenhouse conditions. We identified and analysed lines with insertions of the lipid metabolism genes. Plant lines bombarded with the same transgenes, showed a huge diversity in genotypes and demonstrated the haphazard nature of particle bombardment. Gene stability was observed following crossing of genetically modified lines and in all instances the transgenic material was found to be active in fourth generation plants. None of the transgenic lines showed the phenotype expected (increased growth and yield), but all showed aspects. This could indicate that more than these two genes are affected by the enhanced greenhouse effect, or that neither gene is in fact involved and the phenotypes observed are merely a happy coincidence. It is also possible that the full effect of gene up-regulation was not observed since only one of the lines observed showed ubiquitous expression of the transgenes and all showed some changes in expression of the native thioesterase gene. The lipid metabolism of plants from the four genetic groups (GPAT +, thioesterase+, Dual+ and Null) was analysed and, while alterations in lipid and fatty acid metabolism were observed in all transgenic lines, the differences were highly variable between plant lines of the same group, making an overall analysis of changes difficult. It was also found that the null lines showed as much variation in lipid metabolism as the transgenic lines indicating that chromosomal damage introduced into the plant lines during the original bombardment and tissue culturing process may have had a huge impact on the phenotype of the transgenic plants.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available