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Title: Induction of interleukin-8 in lung epithelial cells by Gram negative bacteria and its modulation by pulmonary surfactant lipids
Author: Alghaithy, Abdulaziz
ISNI:       0000 0004 2748 3962
Awarding Body: Cardiff University
Current Institution: Cardiff University
Date of Award: 2006
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The surfactants did not inhibit IL-8 induced by IL-1[Special character omitted.] in A549 cells suggesting that they act at the level of the LPS receptor complex. Disruption of membrane micro-domains ('rafts') with methyl-[Special character omitted.]-cyclodextrin significantly inhibited bacteria and LPS induced IL-8 in A549 cells. Isolation of membrane raft containing fractions by sucrose density gradient ultra-centrifugation showed that TLR4 is recruited into membrane lipid rafts on cell stimulation with the bacteria or LPS. The surfactants inhibited the bacterial and LPS mediated translocation of TLR4 into raft domains suggesting that their mechanism of action involves inhibition of LPS receptor complex formation in lipid raft domains.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: R Medicine (General)