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Title: Generation and characterisation of anti-C6 monoclonal antibodies in C6-deficient mice : the search for an anti-C6 therapy
Author: Clayton, Lisa Victoria Jane Eynstone
ISNI:       0000 0004 2748 3233
Awarding Body: Cardiff University
Current Institution: Cardiff University
Date of Award: 2006
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For the second approach, monoclonal antibodies were raised against rabbit, rat, mouse and human C6. The two most interesting antibodies were raised against human C6 and inhibited complement-mediated haemolysis in a cell-based assay. Both of these antibodies were species specific, excluding the possibility of testing their therapeutic properties in animal models of complement-mediated disease. Instead, an ex vivo model of cardiopulmonary bypass was established and used to test the ability of these antibodies to block soluble C5b-9 formation. Neither antibody inhibited soluble C5b-9 formation, suggesting that they might be interfering with the insertion of C6 into the cell membrane during MAC assembly.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QH426 Genetics ; QR180 Immunology