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Title: Transcriptional regulation of the human hyaluronan synthase genes : focus on hyaluronan synthase 2
Author: Monslow, James
ISNI:       0000 0004 2747 4361
Awarding Body: Cardiff University
Current Institution: Cardiff University
Date of Award: 2005
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This thesis examines the transcriptional regulation of the human hyaluronan synthase (HAS) genes, with particular focus on hyaluronan synthase 2. The data details the reconstruction of the genomic structures of each HAS isoform for the first time, as well as analysis of the activity of each HAS proximal promoter. The HAS2 promoter region was then investigated in detail, beginning with the reconfiguration and confirmation of the HAS2 transcription initiation site. The transcription factor stimulating protein 1 (Spl) was then identified as an important regulator in the constitutive expression of the HAS2 gene. Finally, the mechanism involved in the induced expression of the HAS2 gene by the pro-inflammatory cytokine interleukin-1 beta was investigated. The study identified that the NF-kB pathway was not solely responsible for the increase in HAS2 transcription, indicating that another, possibly novel mechanism was involved.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available