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Title: The potential role of fish oil supplementation in overcoming sarcopenia
Author: Kamolrat, Torkamol
ISNI:       0000 0004 2745 8708
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 2013
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Fish oil supplementation (containing eicosapentaenoic acid; EPA and docosahexaenoic acid; DHA) may have a potential role in overcoming the age-related decline in skeletal muscle mass and function, termed sarcopenia. The aim of this thesis was to determine whether fish oil supplementation can enhance anabolic signalling, muscle strength, protein metabolism and functional abilities in ageing skeletal muscle. In ageing rats (aged 13 months) the effect of 8-week fish oil (2.5 g/day, consisting of 240 mg EPA and 245 mg DHA) supplementation on skeletal muscle anabolic signalling pathways and changes in lean body mass was determined. The data revealed fish oil supplementation tended (P=0.09) to preserve total lean mass loss and increased whole-body glucose flux and phosphorylation of the PI3K-PDK1-p70s6k pathway in type II glycolytic muscle fibres. There was no effect of supplementation on markers of inflammation. In C2C12 myotubes, the distinct effects of EPA and DHA on protein synthesis, protein breakdown and signalling pathways, information previously unknown, was examined. Data showed that EPA (50 μM) augmented L-leucine stimulated protein synthesis and anabolic signalling. EPA also reduced protein breakdown and catabolic signalling. DHA (50 μM) increased anabolic signalling (specifically p70s6k phosphorylation) but had no effect on any other measures. In healthy older women (aged > 65 years) the effect of fish oil supplementation, 4 g/day fish oil (containing 1.7 g EPA and 0.4 g DHA) daily for 12 weeks, on adaptations in skeletal muscle function in response to resistance training was examined. The data revealed that fish oil supplementation enhanced adaptations in isometric strength, gait speed, and also decreased markers of insulin resistance. Taken together, our findings indicate that fish oil supplementation may have a potential role in overcoming anabolic resistance in ageing skeletal muscle and may be used as a novel intervention for the prevention and treatment of sarcopenia.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available