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Title: Recognition and reduction of adverse effects in research on rodents
Author: Wadham, John Jeremy Beresford
ISNI:       0000 0004 2745 3827
Awarding Body: University of Birmingham
Current Institution: University of Birmingham
Date of Award: 1996
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Routine husbandry systems and standard experimental techniques were assessed to investigate if they were stressful for laboratory rats. They were then compared with alternative methods which had been putatively "refined" to cause less suffering. It was found that rats provided with various environmental improvements were less anxious than rats housed in standard conditions and so a recommendation is made that rats be provided with nesting material whenever possible. Rats undergoing surgery were found to recover quicker if kept as near as possible to homeostasis through careful choice of peri-operative conditions. Intraperitoneal injection appeared less stressful after habituation to handling and so it is recommended that rats be habituated prior to undergoing some experimental procedures. Restraint for periods of 5 to 120 min was stressful and it is recommended that restrainers are redesigned with the needs of both the animal and the procedure in mind. Euthanasia with rising concentrations of carbon dioxide appeared more humane than immersion of the animal in 100% carbon dioxide or ether or than an intraperitoneal injection of sodium pentobarbitone. Considerable concern was raised over the use of intraperitoneal injection of sodium pentobarbitone as a humane method of euthanasia.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available