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Title: The molecular basis of plant cell wall oligosaccharide formation
Author: Götz, Stephan
ISNI:       0000 0004 2741 2910
Awarding Body: University of East Anglia
Current Institution: University of East Anglia
Date of Award: 2012
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The characterisation and identification of glycosyltransferases (GTs) involved in matrix polysaccharide biosynthesis has gained increasing interest as they affect our ability to influence, engineer and manipulate plant cell wall biosynthesis. To date, only a small number of genes coding for matrix polysaccharide biosynthesis-related glycosyltransferases have been identified. Mostly, biochemical proof of activity is still needed for final confirmation of function. To address such problems, this study established an initial biochemical activity testing methodology combined with final gene identification in one approach. A substrate library was established with different sugar nucleotide donor and oligosaccharide acceptor combinations to address a comprehensive range of glycosyltransferase activities. To overcome well-known problems with radioactive assay formats, an APTS (8-Amino- 1,3,6,PyreneTriSulfonic acid) based detection methodology was introduced, which allowed sensitive and flexible analysis of the glycosyltransferases.
Supervisor: Not available Sponsor: John Innes Centre
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available