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Title: Tissue slice cultures of the lung : an in vitro model to study inflammation and anti-inflammatory compounds
Author: Buzzelli , Daniela
ISNI:       0000 0004 2742 1577
Awarding Body: Queen's University Belfast
Current Institution: Queen's University Belfast
Date of Award: 2012
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The aim of the whole project was to establish Precision-Cut Slices (PCS) from rat lung as an in vitro model to study lung inflammation and test the anti-inflammatory and properties of natural occurring Vitamin E and chromanol- derived compounds. Furthermore, the use of the Raman Microscopy will allow investigating the distribution of these compound oxidation products in rat lung tissue sections. In the current investigation, establishment of PCS from rat lung included 1) assessment of viability of rat lung PCS using the MTT and LDH assays and histopathology, and 2) evaluation of responsiveness of the model to Pseudomonas aeruginosa LPS stimulation looking at production of some pro-inflammatory markers such as IL-8, IL-lβ, TNF-α, and NOx. Beside the establishment of the model for the study of lung inflammation, it was also assessed whether natural occurring vitamin E and chromanol-derived compounds exert any anti-inflammatory effect in Precision-Cut Slices from rat lung upon Pseudomonas aeruginosa LPS challenge. The following compounds were used: α- Tocopherol; γ- Tocopherol; γ- Tocotrienol; α- Tocopherol long-chain carboxychromanol (α-13' -COOH); α-Tocopheramine (α-TNH2); γ-Tocopheramine (γ-TNH2). Effects on secretion of pro-inflammatory mediators, such as IL-8, IL-lβ, TNF-α and PGE2, and on expression of cyclooxygenase-2 (COX-2) enzyme were analyzed to evaluate the anti-inflammatory capacities of these compounds in PCS from rat lung. Maps of rat lung sections were also taken for the analysis of the localization of natural occurring vitamin E and chromanol-derived compounds via Raman Microscopy.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available