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Title: Investigation of human papilloma virus infections in prostate cancer
Author: Azzawi, Mous'ab
ISNI:       0000 0004 2740 9906
Awarding Body: Middlesex University
Current Institution: Middlesex University
Date of Award: 2013
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Human papillomavirus (HPV) infections are associated with benign and malignant lesions of the female and male anogenital tract. In the current study, we aimed to investigate the role of high-risk HPVs infection in the pathogenesis of prostate cancer among nations or ethnic groups, in addition to testing the role of homozygosity of arginine form at codon 72 of the p53 gene among prostate cancer patients whose prostate tissues were infected with high-risk HPVs. Formalin-fixed paraffin-embedded tissue samples of 123 primary prostate adenocarcinoma cases and 267 control tissues of benign prostatic hyperplasia were used in the study. Genomic DNA was purified and amplified through MY09/MY11 degenerate primers, GP5+/GP6+ consensus primers, SPF1/2 cocktail of six primers using conventional, multiplex and nested PCR techniques, and subsequently subjected to viral load quantification, genotyping, testing of polymorphism of codon 72 of the p53 gene and apoptosis index assessment by in situ assay. Also, the status of the p53 tumour suppressor gene, p16INK4a transcription factor as well as the E6 protein of the high risk HPVs have been tested by immunohistochemistry in both the study and control groups. High-risk HPVs were detected in 30 of 123 (24.3%) PCa and 16 of 267 (5.9%) BPH samples with positive HPV-DNA. The detection rate of the high-risk HPV infections was 4%, 44% and 29% among the ethnic subgroups from the Middle Eastern, Caucasian, and Afro-Caribbean of the PCa patients. There was no association between the existence of high-risk HPV infections and their viral load in PCa patients and the tumour staging, grading, PSA level and patient survival rate in those patients. Likewise, there was no significant difference in the frequency of p53 Arg 16 homozygosity between the high-risk HPV-positive and the HPV-negative PCa samples. Moreover, it has been found that the existence of the highrisk HPV E6 protein within the PCa samples was independent of the status of the p53 gene, p16INK4a transcription factor, and the apoptosis index in these samples. Our data showed that HPV infections do exist in PCa and BPH samples with different prevalence within ethnic groups with the least occurrence in the Middle Eastern patients. However, the infections with high-risk HPVs are not associated with the prostate cancer grade, stage, patient’s PSA level, and survival rate. Therefore, our data do not support the role of HPV infection in the pathogenesis of prostate carcinoma.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available